BD-PE2337-DDX39B (16Z17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DDX39B (16Z17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2337

产品名称

DDX39B (16Z17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DDX39B

推荐应用

反应种属

Human,Dog,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 319-428 of human DDX39B (NP_004631) produced in E.coli.

稀释度

WB 1:500, IHC 1:500

预测分子量

48.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDX39B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2337) (1:500)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DDX39B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2337) (1:500)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DDX39B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2337) (1:500)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DDX39B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2337) (1:500)

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DDX39B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2337) (1:500)

Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-DDX39B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2337) (1:500)

Western blot analysis of extracts (35ug) from different 6 cell lines lysates by using anti-DDX39B monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX39B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX39B (1:500). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.