BD-PE2322-RAD9A (RAD9) (15G18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RAD9A (RAD9) (15G18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2322

产品名称

RAD9A (RAD9) (15G18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RAD9A

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human RAD9A expression vector

稀释度

WB 1:2000 IHC 1:50

预测分子量

42.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2322, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2322, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2322, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2322, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2322, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A(Cat# BD-PE2322). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.