BD-PE2312-NDUFS2 (16Q2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NDUFS2 (16Q2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2312

产品名称

NDUFS2 (16Q2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

NDUFS2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 205-463 of human NDUFS2 (NP_004541) produced in E.coli.

稀释度

WB 1:2000

预测分子量

49.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (BD-PE2312) Dilution: 1:150

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NDUFS2 mouse monoclonal antibody. (BD-PE2312) Dilution: 1:150

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (BD-PE2312) Dilution: 1:150

Western blot analysis of A549 cell lysate (35ug) by using anti-NDUFS2 monoclonal antibody. Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NDUFS2 mouse monoclonal antibody. (BD-PE2312) Dilution: 1:150

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (BD-PE2312) Dilution: 1:150

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NDUFS2 mouse monoclonal antibody. (BD-PE2312) Dilution: 1:150

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFS2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.