BD-PE2286-FOLH1 (3E3) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FOLH1 (3E3) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2286

产品名称

FOLH1 (3E3) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FOLH1

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500~2000, IF 1:100, IF 1:100

预测分子量

84.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunofluorescent staining of PC-12 cells using FOLH1 mouse monoclonal antibody (BD-PE2286, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FOLH1mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-FOLH1mouse monoclonal antibody.

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOLH1 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-FOLH1 very specifically recognizes FOLH1 antigen on OriGene protein microarray chip.

Western blot of mouse tissue lysates (20ug) from Brain. Diluation: 1:500.

Western blot of cell lysates (35ug) from MDCK. Diluation: 1:500

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOLH1mouse monoclonal antibody.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-FOLH1 (PSMA) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-FOLH1 (PSMA) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-FOLH1 monoclonal antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-FOLH1mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-FOLH1mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FOLH1mouse monoclonal antibody.