BD-PE2273-Purified HER2 (ERBB2) (15I15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Purified HER2 (ERBB2) (15I15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2273

产品名称

Purified HER2 (ERBB2) (15I15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ERBB2

推荐应用

反应种属

Human,Dog,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB:1:500, IHC:1:50, IF:1:100

预测分子量

137.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Anti-ERBB2 mouse monoclonal antibody (BD-PE2273, 3, 1:100) immunofluorescent staining of COLO205 cells.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody at 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) using anti-ERBB2mouse monoclonal antibody. (3, dilution 1:100; heat-induced epito

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ERBB2 monoclonal antibody (1:500).

Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (BD-PE2273, 3, 1:2000).

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody.

Anti-ERBB2 mouse monoclonal antibody (BD-PE2273, 3, 1:100) immunofluorescent staining of MCF7 cells.