BD-PE2258-DFF45 (ICAD) (1X16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DFF45 (ICAD) (1X16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2258

产品名称

DFF45 (ICAD) (1X16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DFFA

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45

稀释度

WB: 1:500, IF (1:100)

预测分子量

36.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene

期货

2周

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

DFF45 antibody (1B10) at 1:500 dilution + Hela cell lysate

Immunofluorescent staining of HeLa cells using anti-DFFA mouse monoclonal antibody (BD-PE2258).

Anti-DFFA mouse monoclonal antibody (BD-PE2258) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY DFFA

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.