OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CDH1 mouse monoclonal antibody (BD-PE2243). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Flow cytometric analysis of living T47D cells, using anti-CDH1 antibody (BD-PE2243, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE2243) (1:200)

Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.

Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.

Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.

Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH1 (1:500).

Western blot of human tissue lysates (15ug) from 5 different tissues (1: Liver, 2: Ovary, 3: Thyroid, 4: Colon, 5: Spleen ). Diluation: 1:500.

IHC staining of FFPE human breast cancer using anti-CDH1 84 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. BD-PE2243 requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE2243) (1:200)

Flow cytometric analysis of living 293T cells transfected with CDH1 overexpression plasmid (Red)/empty vector (Blue) using anti-CDH1 antibody (BD-PE2243). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Western blot analysis of extracts (35ug) from 2 tissue lysates by using anti-CDH1 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.

Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.

Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.

Western blot analysis of extracts (35ug) from 6 cell lines lysates by using anti-CDH1 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE2243) (1:200)

Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.

Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 84 (BD-PE2243) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE2243) (1:200)