RM1382-BRAF mutant V600E (16I10) Rabbit Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BRAF mutant V600E (16I10) Rabbit Monoclonal antibody

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产品基本信息

产品货号

RM1382

产品名称

BRAF mutant V600E (16I10) Rabbit Monoclonal antibody

类别

常规抗体

基因名称

B Raf

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BRAF V600E (NP_004324). The exact sequence is proprietary.

稀释度

WB 1:500

运输及保存条件

-20°C/1 year

宿主

Rabbit

同种型

IgG

背景介绍

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistology staining on Negative Control overexpressed cytosection with rabbit anti BRAF (V600E) C/N RM1382 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit . Positive stain shown with the brown chromogen present. 5 micron sections, 40x magnification

Immunohistology staining on BRAF overexpressed cytosection with rabbit anti BRAF (V600E) C/N RM1382 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit . Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone , 5 micron sections, 40x magnification

Immunohistology staining on BRAF mutant (V600E) overexpressed cytosection with rabbit anti BRAF (V600E) C/N RM1382 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit . Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone , 5 micron sections, 40x magnification