BD-PE2230-BRAF (5B15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BRAF (5B15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2230

产品名称

BRAF (5B15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BRAF

推荐应用

反应种属

Human,Dog,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human BRAF expression vector

稀释度

WB 1:2000

预测分子量

84.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (BD-PE2230)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.