OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-UCHL1 (PGP9.5) mouse monoclonal antibody (BD-PE2188). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded COS-7 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded mouse cerebrum tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded Huh7 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded NIH/3T3 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded rat cerebrum tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:150) (1:600)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded MCF7 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-UCHL1 (PGP9.5) Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2188) (1:600)