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HSPA9 (5D10) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE2176
产品名称
HSPA9 (5D10) Mouse Monoclonal antibody
类别
常规抗体
基因名称
HSPA9
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
稀释度
IF 1:100, FCM 1:100, WB: 1:200
预测分子量
73.5kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSPA9 antibody (BD-PE2176), and then analyzed by flow cytometry.

Anti-HSPA9 mouse monoclonal antibody (BD-PE2176) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 .

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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