BD-PE2174-HSPA9 (13R9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HSPA9 (13R9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2174

产品名称

HSPA9 (13R9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

HSPA9

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IHC 1:50, IF 1:50, FCM 1:100

预测分子量

73.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of Hela cells, using anti-HSPA9 antibody (BD-PE2174), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-HSPA9 antibody (BD-PE2174), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HSPA9 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2174)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2174)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2174)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2174)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2174)

Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody (BD-PE2174).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2174)

Anti-HSPA9 mouse monoclonal antibody (BD-PE2174) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9(Cat# BD-PE2174). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.