BD-PE2171-GZMB (4I4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GZMB (4I4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2171

产品名称

GZMB (4I4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GZMB

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 21-247 of human GZMB(NP_004122) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150, IF 1:100

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GZMB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE2171)

Anti-GZMB mouse monoclonal antibody (BD-PE2171) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GZMB .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GZMB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GZMB(Cat# BD-PE2171). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.