BD-PE2087-FADD (7I13) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FADD (7I13) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2087

产品名称

FADD (7I13) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FADD

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human FADD (NP_003815) produced in HEK293T cell.

稀释度

WB 1:2000, FCM 1:100

预测分子量

23.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-FADD monoclonal antibody at 1:200 dilution.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FADD antibody (BD-PE2087), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FADD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FADD (BD-PE2087). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.