BD-PE2074-B4GALT4 (9P5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

B4GALT4 (9P5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2074

产品名称

B4GALT4 (9P5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

B4GALT4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 40-322 of human B4GALT4(NP_003769) produced in E.coli.

稀释度

WB 1:500, IHC 1:150

预测分子量

39.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-B4GALT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2074) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-B4GALT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2074) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-B4GALT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2074) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B4GALT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B4GALT4 (Cat# BD-PE2074)(1:500). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.