BD-PE2052-LGR5 (7T13) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

LGR5 (7T13) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2052

产品名称

LGR5 (7T13) Mouse Monoclonal antibody

类别

常规抗体

基因名称

LGR5

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:200~2000, IHC 1:100~150 (recommended antigen retrieval buffer: TEE buffer pH 9.0), IF 1:100, FCM 1:100

预测分子量

99.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGR5 mouse monoclonal antibody (BD-PE2052) at 1:100. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Western blot analysis of extracts (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody (left lane: Brain right lane: Testis) (1:200).

Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 at 1:400 with GBI Polink2 Broad HRP DAB detection kit ; pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.

NIH-3T3 (Left lane) or stable expressed LGR5-3T3 (Right lane) cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5 (1:1000).

Western blot analysis of extracts (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody (1:2000). (Lanes: control vector pCMV6-Entry; human LGR4 , LGR5 and LGR6 ; or mouse LGR4, LGR5 and LGR6).

Flow cytometric analysis of stable expression LGR5 HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody (BD-PE2052, 1:100) surface stained on live cells.

Flow cytometric analysis of HEK293T cells transiently transfected with human LGR4 or mouse LGR4, human LGR5 or mouse LGR5, human LGR6 or mouse LGR6, or control vector pCMV6-Entry, using anti-LGR5 antibody (BD-PE2052, 1:100) surface stained on live cells.

Anti-LGR5 mouse monoclonal antibody (BD-PE2052) immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5 (1:100).

Anti-LGR5 mouse monoclonal antibody (BD-PE2052) immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY human LGR5 (1:100).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE2052) (1:150)