BD-PE2034-RGS5 (7N9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RGS5 (7N9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2034

产品名称

RGS5 (7N9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RGS5

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, FCM 1:100

预测分子量

20.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants encoding different isoforms have been identified.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RGS5 antibody (BD-PE2034), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2034)

Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in human brain pericytes. Dilution: 1:200

Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in primary neonatal pig GMCs.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2034)

Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody (BD-PE2034), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5(Cat# BD-PE2034). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.