BD-PE1991-VSNL1 (2C2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

VSNL1 (2C2) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE1991

产品名称

VSNL1 (2C2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

VILIP1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 2-191 of human VSNL1 (NP_003376) produced in E.coli.

稀释度

IHC 1:200

预测分子量

22kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded human skin using anti-VSNL1 15 mouse monoclonal antibody (BD-PE1991) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pre

Immunohistochemical staining of paraffin-embedded human glioma using anti-VSNL1 15 mouse monoclonal antibody (BD-PE1991) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using p

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded human pancreas using anti-VSNL1 15 mouse monoclonal antibody (BD-PE1991) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using

Immunofluorescent staining of HepG2 cells using anti-VSNL1 mouse monoclonal antibody (BD-PE1991, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Confocal immunofluoresce image of primary rat neurons labeled with anti-VSNL1 mouse monoclonal antibody (BD-PE1991, green, 1:100). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (BD-PE1991; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)