BD-PE1979-VBP1 (11N5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

VBP1 (11N5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1979

产品名称

VBP1 (11N5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

VBP1

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human VBP1(NP_003363) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100

预测分子量

22.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-VBP1 mouse monoclonal antibody (BD-PE1979). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VBP1 mouse monoclonal antibody. (BD-PE1979; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-VBP1 mouse monoclonal antibody. (BD-PE1979; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VBP1 mouse monoclonal antibody. (BD-PE1979; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western Blot analysis of HT-29, BT-549 and MOLT-4 cell lysates (35ug) by using anti-VBP1 monoclonal antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VBP1 mouse monoclonal antibody. (BD-PE1979; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-VBP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunofluorescent staining of MDA-MB-231 cells using anti-VBP1 mouse monoclonal antibody (BD-PE1979, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Western blot of mouse tissue lysates (20ug) from Ovary. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VBP1 mouse monoclonal antibody. (BD-PE1979; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-VBP1 mouse monoclonal antibody. (BD-PE1979; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)