BD-PE1963-UBA7 (17I5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

UBA7 (17I5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1963

产品名称

UBA7 (17I5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

UBA7

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human UBA7 (NP_003326) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:500

预测分子量

111.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-UBA7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE1963) (1:500)

Western blot analysis of extracts (35ug) from 4 cell lines by using anti-UBA7 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA7(Cat# BD-PE1963). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.