BD-PE1939-Troponin C (TNNC1) (19Q7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Troponin C (TNNC1) (19Q7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1939

产品名称

Troponin C (TNNC1) (19Q7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TNNC1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human TNNC1 (NP_003271) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

18.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNNC1 mouse monoclonal antibody (BD-PE1939). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Western blot of cell lysates (35ug) from 8 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: MCF7, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12). Diluation: 1:500.

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-TNNC1 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE1939) (1:200)