BD-PE1902-SYP (19I8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SYP (19I8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1902

产品名称

SYP (19I8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SYP

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SYP(NP_003170) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

33.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SYP mouse monoclonal antibody (BD-PE1902). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Confocal immunofluoresce image of primary rat neurons labeled with anti-SYP mouse monoclonal antibody (BD-PE1902, green, 1:100). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20um.

Western blot of mouse tissue lysates (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

Immunohistochemical staining of paraffin-embedded mouse small intestine using mouse anti-SYP 12 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE1902 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Immunohistochemical staining of paraffin-embedded mouse liver using mouse anti-SYP 12 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE1902 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).

Immunohistochemical staining of paraffin-embedded mouse brain using mouse anti-SYP 12 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE1902 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Immunohistochemical staining of paraffin-embedded human brain using anti-SYP 12 mouse monoclonal antibody at 1:800 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1902 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in granular layer and purkinje cells.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded human carcinoid tumor using anti-SYP 12 mouse monoclonal antibody at 1:200 dilution of 0.4mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1902 requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in the tumor cells with surrounding stroma negative.

Immunohistochemical staining of paraffin-embedded human pancreas using anti-SYP 12 mouse monoclonal antibody at 1:200 dilution of 0.4mg/mL detection with Polink2 Broad HRP DAB. BD-PE1902 requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in the Islets of Langerhans with no staining in the glandular cells.

Immunofluorescent staining of SH-SY5Y cells using anti-SYP mouse monoclonal antibody (BD-PE1902, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-SYP mouse monoclonal antibody. (BD-PE1902; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)