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Stat4 (3H6) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE1877
产品名称
Stat4 (3H6) Mouse Monoclonal antibody
类别
常规抗体
基因名称
STAT4
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Recombinant fragment expressed in E.coli corresponding to amino acids 633-652 of human Stat4
稀释度
WB: 1:100, IHC 1:50
预测分子量
85.8kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
Stat4 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-Stat4 (BD-PE1877) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

STAT4 antibody ( 2A2 ) at 1:100 dilution + Hela cell lysate

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Stat4 (BD-PE1877) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Stat4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Stat4. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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