BD-PE1869-SSB (19P19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SSB (19P19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1869

产品名称

SSB (19P19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SSB

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human SSB expression vector

稀释度

WB 1:1000~2000, IF 1:50~100, FCM 1:100, IP 2ug/500ul

预测分子量

46.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.

Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (BD-PE1869).

Figure from citation: Immunoprecipitation of SSB (La) protein level by using anti-SSB antibody in HeLa cells.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-SSB mouse monoclonal antibody (BD-PE1869) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB .

Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody (BD-PE1869), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSB monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB(Cat# BD-PE1869). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.