BD-PE1857-SPG7 (14V7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SPG7 (14V7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1857

产品名称

SPG7 (14V7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SPG7

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) prodduced in E.coli.

稀释度

WB 1:500~2000, IF 1:50~100, FCM 1:100

预测分子量

88.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPG7 antibody (BD-PE1857), and then analyzed by flow cytometry.

Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody (BD-PE1857).

Anti-SPG7 mouse monoclonal antibody (BD-PE1857) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.