BD-PE1811-MAP2K4 (MEK4) (10K8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAP2K4 (MEK4) (10K8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1811

产品名称

MAP2K4 (MEK4) (10K8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAP2K4

推荐应用

反应种属

Human,Dog,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

稀释度

WB 1:500~1000, IHC 1:150, IF 1:50~100, FCM 1:100, IP 2ug/500ul

预测分子量

44.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1811)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1811)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1811)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K4 antibody (BD-PE1811), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1811)

Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (BD-PE1811).

Anti-MAP2K4 mouse monoclonal antibody (BD-PE1811) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4 .

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-MAP2K4 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1811)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4(Cat# BD-PE1811). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.