BD-PE1802-SERPINB4 (16W2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SERPINB4 (16W2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1802

产品名称

SERPINB4 (16W2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SERPINB4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SERPINB4 (NP_002965) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500, IHC 1:150, IF 1:100

预测分子量

44.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

SERPINB4 is also called squamous cell carcinoma antigen 2 (SCCA-2) or leupin. It directly inhibits human granzyme proteolytic activity and that overexpression of SERPINB4 in HeLa cells inhibits recombinant granzyme-induced as well as NK cell-mediated cell death.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded human gastric carcinoma using anti-SERPINB4 6 mouse monoclonal antibody (BD-PE1802) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membrane staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-SERPINB4 6 mouse monoclonal antibody (BD-PE1802) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membrane staining is seen in tumor cells.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINB4 monoclonal antibody at 1:500.

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-SERPINB4 6 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1802 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-SERPINB4 6 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1802 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunofluorescent staining of HepG2 cells using SERPINB4 mouse monoclonal antibody (BD-PE1802, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SERPINB4 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SERPINB4 very specifically recognizes SERPINB4 antigen on OriGene protein microarray chip.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:1000.

Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-SERPINB4 6 mouse monoclonal antibody (BD-PE1802) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membrane staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SERPINB4mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SERPINB4mouse monoclonal antibody.