BD-PE1794-S100A9 (10K14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

S100A9 (10K14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1794

产品名称

S100A9 (10K14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

S100A9

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human S100A9 (NP_002956) produced in SF9 cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

13.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-S100A9 mouse monoclonal antibody (BD-PE1794). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-S100A9 74 mouse monoclonal antibody (BD-PE1794) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear, cytoplasmic, and membrane staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded human lung carcinoma using anti-S100A9 74 mouse monoclonal antibody (BD-PE1794) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear, cytoplasmic, and membrane staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-S100A9 mouse monoclonal antibody. (BD-PE1794; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human melanoma using anti-S100A9 74 mouse monoclonal antibody (BD-PE1794) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear, cytoplasmic, and membrane staining is seen in infiltrating lymphacytes and no staining is tumor cells.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-S100A9 mouse monoclonal antibody. (BD-PE1794; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-S100A9 74 mouse monoclonal antibody (BD-PE1794) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear, cytoplasmic, and membrane staining is seen in macrophages and no staining is tumor cells.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-S100A9 mouse monoclonal antibody. (BD-PE1794; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-S100A9 mouse monoclonal antibody. (BD-PE1794; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human lung using anti-S100A9 74 mouse monoclonal antibody (BD-PE1794) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear, cytoplasmic, and membrane staining is seen in epithelial cells.

Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-S100A9 mouse monoclonal antibody. (BD-PE1794; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-S100A9 mouse monoclonal antibody. (BD-PE1794; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)