BD-PE1777-ROS1 (13R1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ROS1 (13R1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1777

产品名称

ROS1 (13R1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ROS1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1(NP_002935) produced in E.coli.

稀释度

WB 1:2000, IHC 1:5000

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (BD-PE1777; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (BD-PE1777) (Left). The right is HELA cells as negative control (1:2000).

Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using BD-PE1777 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min)

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.