BD-PE1743-RANGAP1 (8N19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RANGAP1 (8N19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1743

产品名称

RANGAP1 (8N19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RANGAP1

推荐应用

反应种属

Human,Dog,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IHC 1:50, IF 1:100, FCM 1:100

预测分子量

63.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1743, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1743, Dilution 1:50)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RANGAP1 antibody (BD-PE1743), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody (BD-PE1743), (Red), compared to a nonspecific negative control antibody , (Blue).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1743, Dilution 1:50)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RANGAP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-RANGAP1 mouse monoclonal antibody (BD-PE1743) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1 .

Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody (BD-PE1743), (Red), compared to a nonspecific negative control antibody , (Blue).

Western blot analysis of extracts (10ug) from a mouse cell line by using anti-RANGAP1 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1(Cat# BD-PE1743). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.