BD-PE1713-PSMD2 (Proteasome 26S S2) (15I14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PSMD2 (Proteasome 26S S2) (15I14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1713

产品名称

PSMD2 (Proteasome 26S S2) (15I14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PSMD2

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PSMD2(NP_002799) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100

预测分子量

100kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD2 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

Anti-PSMD2 mouse monoclonal antibody (BD-PE1713) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMD2 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1713)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD2(Cat# BD-PE1713). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.