BD-PE1710-PSMC3 (16X18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PSMC3 (16X18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1710

产品名称

PSMC3 (16X18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PSMC3

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:50, IF 1:100

预测分子量

49.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PSMC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1710, Dilution 1:50)

Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody (BD-PE1710).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1710, Dilution 1:50)

Anti-PSMC3 mouse monoclonal antibody (BD-PE1710) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.