BD-PE1686-PSMA2 (Proteasome 20S alpha 2) (13R17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PSMA2 (Proteasome 20S alpha 2) (13R17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1686

产品名称

PSMA2 (Proteasome 20S alpha 2) (13R17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PSMA2

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 50-234 of human PSMA2(NP_002778) produced in E.coli.

稀释度

WB 1:2000, IHC 1:500

预测分子量

25.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PSMA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1686)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA2(Cat# BD-PE1686). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.