BD-PE1659-ERK3 (10L16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ERK3 (10L16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1659

产品名称

ERK3 (10L16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAPK6

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3

稀释度

WB: 1:1000, IHC 1;50

预测分子量

82.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-ERK3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).