BD-PE1608-PIK3C2A (14B4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PIK3C2A (14B4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1608

产品名称

PIK3C2A (14B4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PI 3 Kinase Class 2A

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

预测分子量

190.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1608)

Western blot analysis of HT29 cell lysate (35ug) by using anti-PIK3C2A monoclonal antibody. Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1608)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1608)

E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) were separated by SDS-PAGE and immunoblotted with anti-PIK3C2A.