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PFKP (1R11) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE1603
产品名称
PFKP (1R11) Mouse Monoclonal antibody
类别
常规抗体
基因名称
PFKP
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.
稀释度
WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100
预测分子量
85.4kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2. [supplied by OMIM]
期货
2周
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of Hela cells, using anti-PFKP antibody (BD-PE1603), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-PFKP antibody (BD-PE1603), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

Anti-PFKP mouse monoclonal antibody (BD-PE1603) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1603)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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