BD-PE1515-MMP7 (9E16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MMP7 (9E16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1515

产品名称

MMP7 (9E16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MMP7

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 95-267 of human MMP7 (NP_002414) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

19.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MMP7 mouse monoclonal antibody (BD-PE1515). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MMP7 mouse monoclonal antibody. (BD-PE1515; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MMP7 mouse monoclonal antibody. (BD-PE1515; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7). Diluation: 1:500

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MMP7 mouse monoclonal antibody. (BD-PE1515; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human gastric tumor using anti-MMP7 68 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1515 requires heat-induced epitope retrieval with EDTA pH 8.0 in a presure cooker for 3 minutes at 110C. The composit image shows two tumors with membranous and cytoplasmic staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-MMP7 68 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1515 requires heat-induced epitope retrieval with EDTA pH 8.0 in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MMP7 mouse monoclonal antibody. (BD-PE1515; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)