BD-PE1508-Purified KI67 (MKI67) (2G18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Purified KI67 (MKI67) (2G18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1508

产品名称

Purified KI67 (MKI67) (2G18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MKI67

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.

稀释度

IHC: 1:300

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MKI67 mouse monoclonal antibody. (BD-PE1508; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded normal human tonsil tissue using anti-MKI67 mouse monoclonal antibody. (BD-PE1508 at 1ug/ml for 30 min at RT; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 20min; )

Western Blot analysis of HT-29 and NCI-H322M cell lysates (35ug) by using anti-KI67 monoclonal antibody

Western Blot analysis of MOLT-4 and MDA-MB231 cell lysates (35ug) by using anti-KI67 monoclonal antibody

Immunohistochemical staining of paraffin-embedded of 3 human lung cancer using anti-Ki67 07 mouse monoclonal antibody at 1:1000 dilution of 0.5mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1508 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human lung cancer all show strong nuclear staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded of 3 human colon cancer using anti-Ki67 07 mouse monoclonal antibody at 1:1000 dilution of 0.5mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1508 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human colon cancer all show strong nuclear staining in the tumor cells.

Immunofluorescent staining of MDA-MB-231 cells using anti-KI67 mouse monoclonal antibody (BD-PE1508, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded mouse colon tissue using anti-MKI67 mouse monoclonal antibody. (BD-PE1508; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (BD-PE1508; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)