BD-PE1466-EPCAM (13N9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EPCAM (13N9) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE1466

产品名称

EPCAM (13N9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

EPCAM

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:4000, IHC 1:100~200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EPCAM mouse monoclonal antibody (BD-PE1466). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPCAM mouse monoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of LOVO cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPCAMEPCAMmonoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EPCAM mouse monoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EPCAMmouse monoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-EPCAM mouse monoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-EPCAM monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Western blot analysis of HEK293 cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-EPCAMmouse monoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of COLO205 cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-EPCAM mouse monoclonal antibody. (BD-PE1466; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)