BD-PE1462-EPCAM (10A14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EPCAM (10A14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1462

产品名称

EPCAM (10A14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

EPCAM

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.

稀释度

WB: 1:1000-1:4000, IF 1:100, FCM 1:100, IHC: 1:150-1:500

预测分子量

32.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of live HEK293 cells transfected with EPCAM ORF , using anti-EPCAM antibody (BD-PE1462 at 1:100, Red), compared to IgG1 isotype control (Black).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPCAM (1:4000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Flow cytometric analysis of live HT29 cells, using anti-EPCAM antibody (BD-PE1462 at 1:100, Red), compared to IgG1 isotype control (Black).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (BD-PE1462)

Flow cytometric analysis of live OVCAR-4 cells, using anti-EPCAM antibody (BD-PE1462 at 1:100, Red), compared to IgG1 isotype control (Black).

Immunofluorescent staining of HeLa cells using anti-EPCAM mouse monoclonal antibody (BD-PE1462).