BD-PE1452-LIMK1 (LIM Kinase 1 ) (4Z5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

LIMK1 (LIM Kinase 1 ) (4Z5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1452

产品名称

LIMK1 (LIM Kinase 1 ) (4Z5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

LIM Kinase 1

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

72.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1452)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIMK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1452)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1452)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIMK1 antibody (BD-PE1452), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1452)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1452)

Anti-LIMK1 mouse monoclonal antibody (BD-PE1452) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1452)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1 (Cat# BD-PE1452). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.