BD-PE1446-Galectin 3 (LGALS3) (13S7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Galectin 3 (LGALS3) (13S7) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE1446

产品名称

Galectin 3 (LGALS3) (13S7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

LGALS3

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:2000, IHC 1:100~200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGALS3 mouse monoclonal antibody (BD-PE1446). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Western blot analysis of LOVO cell lysate (35ug) by using anti-LGALS3 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Western blot analysis of MALME3M cell lysate (35ug) by using anti-LGALS3 monoclonal antibody.

Western blot analysis of UACC62 cell lysate (35ug) by using anti-LGALS3 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-LGALS3 mouse monoclonal antibody (BD-PE1446, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-LGALS3 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGALS3 mouse monoclonal antibody. (BD-PE1446, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)