Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE1436) (1:150)

LAG3 stable cells was stained by anti-LAG3 (BD-PE1436, red) or IgG isotype control (blue) and analyzed by flow. Control IgG and LAG3 were diluted 1:100

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LAG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE1436) (1:150)

Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (BD-PE1436) (1:100).

Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-LAG3 antibody (BD-PE1436). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

Western blot analysis of extracts (35ug) from 2 cell lines (Human PBMC treated with 10ug/ml PHA for 72h (Right)/untreated (Left)) lysates by using anti-LAG3 monoclonal antibody (1:150).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3 (Cat# BD-PE1436)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.