BD-PE1431-KRT19/CK19 (4V5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

KRT19/CK19 (4V5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1431

产品名称

KRT19/CK19 (4V5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KRT19

推荐应用

反应种属

Human,Dog

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:1000, IHC 1:50, IF 1:100

预测分子量

43.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT19 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT19 very specifically recognizes KRT19 antigen on OriGene protein microarray chip.

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-KRT19mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded liver tissue using anti-KRT19mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-KRT19mouse monoclonal antibody.

HEK293T were transfected with 55 different plasmids of CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 antibody. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative (1:500).

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-KRT19mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-KRT19mouse monoclonal antibody.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CK19 monoclonal antibody at 1:500.

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-KRT19mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-KRT19mouse monoclonal antibody.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-KRT19mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-KRT19 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-KRT19mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-KRT19 (CYTOKERATIN 19) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1431 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunofluorescent staining of MDCK cells using anti-CK19 mouse monoclonal antibody (BD-PE1431, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded breast tissue using anti-KRT19 mouse monoclonal antibody.