BD-PE1429-CK19 (Keratin 19) (14Y17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CK19 (Keratin 19) (14Y17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1429

产品名称

CK19 (Keratin 19) (14Y17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KRT19

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19

稀释度

WB 1:1000, IHC 1:50 -1:150

预测分子量

43.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1429, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT19 mouse monoclonal antibody (BD-PE1429) at 1:150 dilution at 1:150 dilution.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1429, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1429, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.