BD-PE1427-KRT8/CK8 (2Z14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

KRT8/CK8 (2Z14) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE1427

产品名称

KRT8/CK8 (2Z14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KRT8

推荐应用

反应种属

Human,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500~1000, IHC 1:50, IF 1:50~100

预测分子量

53.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-KRT8 (CYTOKERATIN 8) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1427 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8 (1:500).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-KRT8 (CYTOKERATIN 8) mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1427 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunofluorescent staining of A549 cells using anti-CK8 mouse monoclonal antibody (BD-PE1427, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-KRT8 (CYTOKERATIN 8) mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1427 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot of mouse tissue lysates (20ug) from Colon. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody at 1:500.

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT8 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT8 very specifically recognizes KRT8 antigen on OriGene protein microarray chip.

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-KRT8mouse monoclonal antibody.