BD-PE1422-CK8 (Keratin 8) (5Q5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CK8 (Keratin 8) (5Q5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1422

产品名称

CK8 (Keratin 8) (5Q5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KRT8

推荐应用

反应种属

Human,Dog,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

稀释度

WB 1:2500~5000, IF 1:100

预测分子量

53.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

Anti-KRT8 mouse monoclonal antibody (BD-PE1422) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.