BD-PE1407-JUN (16R18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

JUN (16R18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1407

产品名称

JUN (16R18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

c-Jun

推荐应用

反应种属

Human,Mouse,Rat,Monkey,Dog

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human JUN (NP_002219) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:50~100, IF 1:100

预测分子量

35.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-JUN mouse monoclonal antibody (BD-PE1407). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-JUN monoclonal antibody. (BD-PE1407; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-JUN mouse monoclonal antibody. (BD-PE1407; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-JUN monoclonal antibody .

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-JUN mouse monoclonal antibody. (BD-PE1407; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-JUN mouse monoclonal antibody. (BD-PE1407; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-JUN mouse monoclonal antibody (BD-PE1407, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunofluorescent staining of HeLa cells using JUN mouse monoclonal antibody (BD-PE1407, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-JUN mouse monoclonal antibody. (BD-PE1407; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)