BD-PE1387-IDO1 (15X3) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IDO1 (15X3) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1387

产品名称

IDO1 (15X3) Mouse Monoclonal antibody

类别

常规抗体

基因名称

IDO1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human IDO1(NP_002155) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

45.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. [provided by RefSeq, Feb 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-IDO1 mouse monoclonal antibody (BD-PE1387). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-IDO1 mouse monoclonal antibody. (BD-PE1387; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human spleen using anti-IDO1 26 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1387 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The spleen shows very few cells staining in the red pulp with strong nuclear, membrane, and cytoplasmic staining.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded human tonsil using anti-IDO1 26 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1387 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tonsil shows strong membrane and cytoplasmic in the germinal center and rare strong nuclear, membrane, and cytoplasmic staining in the non germinal centers.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-IDO1 mouse monoclonal antibody. (BD-PE1387; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human placenta using anti-IDO1 26 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1387 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The placenta shows strong membrane and cytoplasmic in the endothelial cells.

Immunofluorescent staining of MCF-7 cells using anti-IDO1 mouse monoclonal antibody (BD-PE1387, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Western blot of mouse tissue lysates (20ug) from Brian. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IDO1 mouse monoclonal antibody. (BD-PE1387; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)