BD-PE1385-IDO1 (12A14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IDO1 (12A14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1385

产品名称

IDO1 (12A14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

IDO1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human IDO1(NP_002155) produced in HEk293T cell.

稀释度

WB 1:200~2000, IHC 1:150, IF 1:100

预测分子量

45.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. [provided by RefSeq, Feb 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC), human IDO1 plasmid (hIDO1), human IDO2 plasmid (hIDO2) using anti-IDO1 antibody BD-PE1385(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1385)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-IDO1 mouse monoclonal antibody (BD-PE1385) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1 .